To study anticonvulsant activity of aqueous extract of Piper nigrum and Cinnamomum zeylanicum in MES and PTZ induced animal model. Anticonvulsant activity was evaluated by maximal electroshock (MES) and pentylenetetrazole (PTZ)-induced seizures in wistar albino rats. In MES model, 150 mA current for 0.2 s was given through ear electrodes to induce convulsions in rats. The duration of tonic extension of hind limb was used as the end point, namely, prevention or decrease in the duration of hind limb extension was considered as a protective action. In the PTZ model, the anticonvulsant property of Piper nigrum and Cinnamomum zeylanicum was assessed by its ability to delay the onset of myoclonic spasm and clonic convulsions produced by intraperitoneal administration of PTZ. In the MES model, Piper nigrum and Cinnamomum zeylanicum reduced all the phases of convulsion. Piper nigrum and Cinnamomum zeylanicum at a dose of 500 mg/kg significantly reduced extensor phase (P<0.001, P<0.01). Combination of both extracts (250+250 mg/kg) reduced extensor phase (P<0.01). In PTZ-induced model, the administration of the both extract at dose of 500 mg/kg,30 min prior to injection of PTZ significantly delayed the onset of clonic seizure (P<0.01). Combination doses of both extract ( 250+250 ) mg/kg exert significant protective ( P<0.01) effect on PTZ-induced convulsions. Standard drug levetiracetam at a dose of 1200 mg/kg showed much delayed onset of clonic seizure. Piper nigrum and Cinnamomum zeylanicum showed anticonvulsant activity in MES and PTZ induced animal model.
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